TNF and migraine disorder: On the other hand, in experimental model of dietary migraine, the pain duration was prolonged because of the disruption of immunopeptidergic network and subsequent dysbiosis of gut microbiota after the application of antibiotic treatment in nitro-glycerin-induced acute migraine-like pain in mice [44] IHowever, the pain prolongation completely disappeared when the effect of TNF-α was blocked through genetic depletion of TNF-α and intra-spinal trigeminal nucleus caudalis injunction of TNF-α antagonist [45].