Recently, it was reported that mannose treatment was associated with the activation of AMPK in osteosarcoma cells.20 Here, we also found that in A375 cells, mannose alone or together with CCCP/iron greatly increased AMPK phosphorylation at Thr172, which was associated with the phosphorylation of the AMPK downstream factor ACC (Fig. 3a). The gene discussed is PRKAB1; the disease is osteosarcoma.