GSDME and melanoma: In a previous study, we reported that carbonyl cyanide m-chlorophenyl hydrazine (CCCP) plus iron induced GSDME-dependent pyroptosis in melanoma cells.14 In a subsequent study, we found that mannose, a C-2 epimer of glucose, dramatically reversed CCCP/iron-induced pyroptosis (including pyroptotic morphology, GSDME cleavage, and lactate dehydrogenase (LDH) release) in different melanoma cell lines (Fig. 1a–c).