To test the functions of AKAP150 in the vlPAG in CPA, we downregulated AKAP150 in the vlPAG using CMV-AKAP150-shRNA, CamkIIα-AKAP150-shRNA, and Vgat1-AKAP150-shRNA in WT mice. The gene discussed is CAMK2A; the disease is congenital primary aphakia.