To further assess TPI importance for ROS production, we utilised CRISPR/Cas9-based editing to disrupt Pkm2 and Tpi (Supplementary Fig. 7a–c) in NB4 cells, a promyelocytic leukaemia cell line that differentiates to maturate neutrophils upon exposure to all-trans retinoic acid (ATRA)28. Here, PKM is linked to acute promyelocytic leukemia.