To further investigate the in vivo role of PD-1H in MMP-13-driven MM bone disease, we injected either PBS, EV-transduced 5TGM1-GFP-Luc or MMP-13-overexpressing (OE) 5TGM1-GFP-Luc into Pd-1hwtRag2-/- and Pd-1h-/-Rag2-/- mice and compared bone lesion progression by micro-qCT. Here, VSIR is linked to Miyoshi myopathy.