BCKDK and breast cancer: To further investigate the mechanism by which BCKDK promotes the dissociation of AJs, the formation of FAs, and the migration of breast cancer cells, we carefully measured the protein levels of talin1 and talin2 when overexpressing BCKDK by western blotting and found that the BCKDK expression levels were significantly upregulated and that the knockdown of BCKDK decreased talin1 (Fig. 3A, B).