To investigate if this open chromatin state has functional significance, we rechallenged functional memory mice with YUMM-OVA or YUMM-GFP33/66 melanoma cells (day 43) and sorted CD8+ T cells one day after tumor rechallenge to measure cytokine production (Fig. 5G and SI Appendix, Fig. S4B). This evidence concerns the gene CD8A and neoplasm.