SLC4A10 and neuroblastoma: Two days post-transfection into the fast-growing mouse neuroblastoma cell line N2a, cells were fixed with 4% PFA and stained with an antibody directed against an N-terminal epitope of SLC4A10 as described previously and with the lectin WGA to label glycan structures associated with the plasma membrane.22,40 As expected, cells transfected with the wild-type SLC4A10 construct displayed a predominant labelling at the plasma membrane, whereas the SLC4A10 p.(Lys577Met;Asn1103Ile) variant protein showed a predominant intracellular localization (Supplementary Fig. 6A).