At older age (50 weeks old), Ldlr-/-.Leiden mice also exhibited increased astrogliosis in the hypothalamus compared to C57BL6/J mice, which is consistent with the obesity-induced hypothalamic injury extensively described in literature (Thaler et al., 2012; Guillemot-Legris and Muccioli, 2017). This evidence concerns the gene LDLR and obesity due to melanocortin 4 receptor deficiency.