RT-qPCR analysis validated these findings, demonstrating increased expression of genes that are normally upregulated by EWSR1-WT1 in the recurrent/metastatic tumors (LCK, TRIM67, CCL25, and CAMK2A), while finding reduced expression of genes normally downregulated by EWSR1-WT1 in the recurrent/metastatic tumors (COL12A1, IGF1, TGFBR2, and ADGRA2) (Figure 4(d)). This evidence concerns the gene CCL25 and metastatic neoplasm.