Third-generation EpCAM-CAR-T cells were cytotoxic to target cells and induced lysis in them in an EPCAM-dependent manner leading to notable regression of tumor growth and secretion of cytotoxic interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in the MDA-MB-231 model. Here, EPCAM is linked to neoplasm.