TUBB and rapid-onset childhood obesity-hypothalamic dysfunction-hypoventilation-autonomic dysregulation syndrome: We selected 2 genes that were each significantly up (SPSPON and CPXM2) or down regulated (TuBB and RPS4Y1) in the pre-adipocytes derived from the patient with ROHHAD compared with the control: tissue-derived subcutaneous adipocytes and using RT-q PCR, successfully validated the changes we had observed with the RNAseq data (Supplementary Figure S1).