We recorded the following data: BTNL8 expression pre-treatment and at 3/6 months post-treatment, HPV negative conversion ratio, regression rate of low-grade squamous intraepithelial lesions (LSIL), incidence of adverse reactions, complication rate, serum inflammatory factors, persistence of HPV positivity, LSIL residue or recurrence, and incidence of high-grade cervical intraepithelial lesions (HCIL).<h4>Results</h4>Patients with HPV infection exhibited higher BTNL8 expression than healthy individuals. Here, BTNL8 is linked to squamous cell intraepithelial neoplasia.