PCa cell-derived EVs facilitated the activity of alkaline phosphatase (ALP) and mineralization of extracellular matrix and continuously upregulated the levels of RUNX2, ALP, alpha-1 type 1 collagen, and osteocalcin by regulating RUNX2 to induce the osteogenic differentiation of hBMSCs. The gene discussed is RUNX2; the disease is posterior cortical atrophy.