• EVs RNA were richer than RNA from cell pellets in urine.• PCa vs. HC: In comparison to HC, both of the GS6 and GS7+ PCa groups had significantly higher expression of PCA3, while only the GS7+ group had significantly higher expression of ERG (no difference between the 2 GS groups).• No significant difference in KLK3 expression between any of the groups. This evidence concerns the gene ERG and posterior cortical atrophy.