studied the safeguard role of H3K27me3 and EZH2 in a rat DKD model and indicated that in rat renal mesangial cells, TGF-β downregulated the expression of EZH2, decreased the enrichment of the epigenetic repressive mark H3K27me3 at the fibrotic gene promoter (e.g., Serpin family E member 1 (Serpine1) and C-C motif chemokine ligand 2 (Ccl2), and increased fibrosis protein expression and renal fibrosis (97). The gene discussed is SERPINE1; the disease is diabetic kidney disease.