For total Tg concentration < 50 ng/mL, the LCA-reactive Tg ratio could not distinguish between healthy volunteers, patients with benign tumours, and patients with thyroid carcinoma without metastasis but, for total Tg concentration > 200 ng/mL, the calculated parameter could discriminate between benign thyroid tumours and thyroid carcinoma without metastasis or with lymph node metastasis. The gene discussed is TG; the disease is benign neoplasm.