We examined the association of variants with a minor allele frequency (MAF) of <0.001 in 22 moderate- to high-penetrance CRC genes.2 For the recessive CRC genes MUTYH, NTHL1, MSH3, and MBD4, we assessed the risk of CRC associated with biallelic or monoallelic carriers. This evidence concerns the gene MBD4 and colorectal carcinoma.