Inhibition of S6 phosphorylation by the GCN2i was also observed at the periphery of colon cancer spheroid indicating that mTORC1 pathway is also repressed in proliferating cells of 3D models and confirmed in GCN2 silenced cells (Fig. 5H and Fig. S7D). Here, EIF2AK4 is linked to malignant colon neoplasm.