Further analysis indicated that the AEW‐induced chronic itch increased the proportion of activated GPER+ neurons in the TG of male mice (Vehicle vs. AEW, p < 0.05; Figure 3F) and female mice (Vehicle vs. AEW, p < 0.01; Figure S4C), indicating that these neurons are involved in the modulation of AEW‐induced chronic itch. This evidence concerns the gene GPER1 and Pruritus.