The missense mutation was the primary type of genetic alteration, and R204*/G in the UCH domain, which was detected in three cases of UCEC, two cases of READ, and one case of COAD tumors, can induce a truncating mutation, translation from Arginine (R) to stop codon or Glycine (G) at 204 sites of USP28 protein. This evidence concerns the gene USP28 and colon adenocarcinoma.