In contrast to the effects of an early Gata3 deletion on T cell priming, a late post-developmental deletion of Gata3 did not affect the relative proportions of IFNγ+IL-17A+ or T-bet+RORγt+ ‘pathogenic’ Th17 cells in the CNS or periphery, but still prevented the development of encephalomyelitis symptoms. Here, IFNG is linked to encephalomyelitis.