A study evaluated the effects of Huaier in mouse chemically-induced HCC model, implying the inhibition of Akt/mTOR pathway as a potential factor in mediating the observed therapeutic efficacy of elevated CD4+, CD8+ cells and CD4+/CD8+ ratio together with downregulated Treg, IL-10 and TGF-β (75). This evidence concerns the gene CD8A and hepatocellular carcinoma.