We further tested the efficacy of the triple blockade of Ly6C/PD-1/CTLA-4 in a clinically relevant autochthonous lung cancer GEMM (KrasLSL-G12D/p53fl/fl) where tumors are induced through intratracheal administration of adenoviral-Cre recombinase (23, 48). The gene discussed is CTLA4; the disease is lung carcinoma.