Evaluation of cytokine changes in the tumor microenvironment by ELISA-based analysis of tumor lysates demonstrated that combined blockade elevated the levels of cytokines IFN-γ, TNF-α, IL4, M-CSF, GM-CSF, and CCL2, while it suppressed the immune suppressive cytokine TGF-β (Figure S1A). The gene discussed is TNF; the disease is neoplasm.