PPARA and obesity due to melanocortin 4 receptor deficiency: Moreover, the H diet increased Nfe2l2 mRNA expression ↑44% (Figure 7A) and increased the signal intensity of Nrf2 nuclear localization ↑48% (Figure 8), these could be due to the development of steatosis grade I, insulin resistance, and obesity in H group.Moreover, Ppara mRNA expression increased ↑80% (Figure 7C) and PPARα nuclear translocation ↑112% (Figure 9) were higher in H rats compared to those of the S group, as expected in murine steatosis models, where Ppara overexpression is a defense mechanism of the liver to delay NAFLD progression to NASH [40].