CEACAM5 and hepatocellular carcinoma: Di H. et al. used a multiplex profiling assay (NAISA, nanozyme-assisted immunosorbent assay) to evaluate with high accuracy the presence of GPC-3 in HCC along with EV proteins, such as CD63 (cluster of differentiation 63), CEA (carcinoembryonic antigen), PD-L1 (programmed cell death ligand (1) and HER2 (human epidermal growth factor receptor (2) [29].