Representative TEM image of sEVs isolated from HCC serum are reported (Figure 4b, scale bar: 0.1 μm) Evaluation of 3 controls and 6 HCC cases via Western Blot identified sEVs containing two GPC-3 domains recognized by individual antibodies (Ab): one that recognized the C-terminal domain, and one that recognized the N-terminal domain (Figure 4c,d). This evidence concerns the gene GPC3 and hepatocellular carcinoma.