TNFSF10 and neoplasm: To determine what immune-cell-secreted molecules were most likely contributing to tumor cell pyroptosis, we probed for a downstream mediator of pyroptotic death gasdermin B expression in tumor cells treated with a vehicle-only control (DMSO), 1 μM elraglusib, 100 ng/mL IFN-γ, 250 ng/mL IFN-γ, 1 ng/mL TNF-α, and 1 ng/mL TRAIL (Figure 1L).