In 3 months old mice, Western blot analysis showed that Vps4a protein levels were significantly decreased in Vps4afl/fl Myh6 mice compared to Vps4afl/fl mice, while the expressions of markers for heart failure, such as p-AKT and Myh7, increased (Figure 4A,B and Figure S2A–D). Here, AKT1 is linked to heart failure.