To understand the upstream molecular mechanisms involved in miR-204-5p repression in thyroid cancer, miR-204-5p and TRMP3 expressions were evaluated after 5′-aza-2-deoxycytidine, a demethylating agent, and MEK, BRAF, PI3K or IGF-1R inhibitors treatments. This evidence concerns the gene IGF1R and thyroid gland carcinoma.