CCL20 and cancer: M1 macrophages cultured without activation factors (w/o) after interaction with the cancer cell spheroid showed increased CCL20 (from (w/o) M1 3 to S + (w/o) M1 15 pg/mL, p = 0.0013) and CXCL1 (from 0.6 to 1.5 ng/mL, p = 0.0032) levels, decreased CCL3 (from 1.5 to 0.2 ng/mL, p = 0.0205) and CXCL13 (from 0.20 to 0.11 ng/mL, p = 0.0144) (Figure S5b).