The increase in chemokines such as CCL20 (from 4 to 33 pg/mL, p = 0.0010), CX3CL1 (from 64 to 143 pg/mL, p = 0.0001), CXCL1 (from 1.2 to 3.6 ng/mL, p = 0.0010), and CXCL10 (from 9 to 18 ng/mL, p = 0.0061) in the S + M2→M1 culture medium can be attributed to the interaction of 4T1/GFP cancer cells with IFNγ/Pam3SCK4 because an increase in these chemokines was observed in spheroids cultured in the presence of IFNγ/Pam3SCK4 without macrophages (Figure 5a). This evidence concerns the gene CXCL1 and cancer.