Earlier, we found that the CpG sites proximal to the ASFMR1 transcription start site within FMR1 intron 2, which we named FREE3 [16], were hypomethylated in FXS lymphoblast cell lines lacking FMRP, with reduced ASFMR1 expression compared with the controls and UFM cell lines [16]. This evidence concerns the gene FMR1 and fragile X syndrome.