After that, the influence from CST3 to TGF-β1 was also detected by qPCR and Western blotting, as shown in Figure 3G,H, in the condition of co-culture, and overexpressing CST3 in CRC cells could inhibit the mRNA and protein expression of TGF-β1 in M2 macrophages, as well as the secretion. This evidence concerns the gene TGFB1 and colorectal carcinoma.