Subsequently, the IHC experiment demonstrated that, compared with the AOM/DSS group, the MC-LR/AOM/DSS group showed more macrophage recruitment by detecting markers of macrophages, F4/80 (Figure 4C), and more M2 macrophages infiltration in CRC tissues by detecting markers of the M2 macrophage, CD163 and CD206 (Figure 4D,E). This evidence concerns the gene MRC1 and infectious otitis media.