As the predicted relationship between TGF-β1 and CST3 by IPA, to further determine whether the reduced expression of CST3 was related to the increased expression of TGF-β1, we detected the expression of CST3 by qPCR and Western blotting in CRC cells from the co-culture system under the exposure of MC-LR and supplemented with a TGF-β1-neutralizing antibody. The gene discussed is CST3; the disease is colorectal carcinoma.