To further explore the molecular mechanism of ICI-induced thyroid dysfunction, we profiled changes in transcription induced by monoclonal antibodies targeting programmed cell death-1 (αPD-1) and programmed cell death-ligand 1 (αPD-L1) in a non-small cell lung cancer (NSCLC) murine model using RNA sequencing. The gene discussed is CD274; the disease is non-small cell lung carcinoma.