In this study, we investigated the effect of Nudt2 knockdown on mTROC1 downstream targets, such as p-P70 s6 kinase, but we did not see any change in these proteins’ expression level, which indicates that Nudt2 knockdown has no effect on mTROC1 activity in melanoma and the effect of Nudt2 on anchorage-independent growth in melanoma is not mediated by mTROC1 activity. Here, NUDT2 is linked to melanoma.