To confirm that the anti-tumor activity of CAR19-iNK-like cells was mediated via NK signaling, we measured NK cell activation markers, including CD161, CD158, and Nkp30, upon exposure of the cells to PKH67-labeled target tumor cells using the gating strategy shown in Figure 6B. Figure 7A–C shows that exposure of NK-like cells to different tumor cells yielded different activation patterns. The gene discussed is KLRB1; the disease is neoplasm.