To determine whether the reduced degranulation of iNKT cells affected cytokine production, we stimulated iNKT cells with αGC-unloaded/loaded THP-1 CD1d cells and analyzed the supernatant for secreted IFN-γ and IL-4 levels after 4 days of culture, in the presence/absence of melanoma cells. The gene discussed is IL4; the disease is melanoma.