WT1 and acute lymphoblastic leukemia: In total, 34 hematologic cell lines were analyzed for expression of WT1 mRNA using RT-PCR, 30 of which originate from malignant blasts of adult or pediatric patients with various FAB subtypes of AML or with CML in blast crisis, three cell lines were derived from blasts of adult or pediatric patients with ALL and one cell line, U-937, was generated from an adult patient with histiocytic lymphoma (also see Table S1).