Flow cytometric analysis of intracellular cytokine production revealed a greatly increased accumulation of CD4+ and CD8+ IL-4+ T cells in the draining lymph nodes of LY2-injected HNSCC tumor-bearing mice compared to non-tumor-bearing mice, but not in the MOC2 HNSCC model (Figure 5A). This evidence concerns the gene IL4 and head and neck squamous cell carcinoma.