We identified several proteins previously reported in ESCC including PDPN, POSTN, TOP2A, MMP12, UBE2C and PLAU, which were overexpressed and CRNN, SPRR3, SORBS2, RHCG, ECM1, and TGM3, which were downregulated. This evidence concerns the gene RHCG and esophageal squamous cell carcinoma.