CD4 and neoplasm: Gating analysis of the flow cytometry data reveals a significant accumulation of CD3+HLA-DR+ cells in the tumor compared with PBMC and TDLN (47.17 ± 24.33%, vs. 24.57 ± 19.33% and 21.78 ± 21.65%, respectively) see Figure 3A. Furthermore, CD3+CD4+Treg+HLA-DR+ cells are significantly accumulated in the tumor compared with TDLN (56.83 ± 13.36% vs. 14.84 ± 12.10%, respectively), see Figure 3B.