Gating analysis of the flow cytometry data reveals a significant accumulation of CD3+CTLA-4+ cells in the tumor compared with TDLN (31.15 ± 27.01% vs. 14.19 ± 25.74%, respectively) see Figure 4A. Furthermore, CD3+CD4+CTLA-4+ cells are accumulated in the tumor compared with PBMC (18.63 ± 6.51% vs. 2.12 ± 2.19%, respectively), see Figure 4B. Lastly, CD3+CD4+Treg+CTLA-4+ cells are significantly accumulated in the tumor compared with PBMC (35.8 ± 8.91% vs. 0.94 ± 1.72%, respectively), see Figure 4C. The gene discussed is CTLA4; the disease is neoplasm.