Given that the AKT/mTOR signaling pathway is a key autophagy regulator in many pathophysiological processes associated with kidney disease [48,49], we used Western blotting to detect the activation of the AKT/mTOR pathway in the renal tissue, and the results show that the phosphorylation levels of AKT and mTOR were significantly decreased by adenine in the rat kidneys. This evidence concerns the gene AKT1 and kidney disorder.