The presence of CGRP-ir neurons in orthotopic oral cancer HSC-3 and OSC-20 xenografts was confirmed using multiplex immunofluorescence staining for CGRP, GAP43 (marker of sprouting neurons), SOX10 (expressed in Schwann cell nuclei), PECAM (blood vessel marker), LYVE1 (lymphatic vessel marker) and TRP63 isoforms (epithelial cells) (Figure 1). This evidence concerns the gene CALCA and lip and oral cavity carcinoma.