CHAT and colitis: Regarding the analysis of the nuclear area of ChAT-ir neurons, no difference (p > 0.05) was observed between the Sham (104.8 ± 2.4 μm2), Colitis (102.0 ± 2.5 μm2), and Butyrate (109.1 ± 2.7 μm2) (Figure 7E).