CHAT and colitis: Myenteric ChAT-ir neurons showed a reduction in CTCF of 21.0% in the Colitis group (1,530,379 ± 52,098) when compared to the Sham group (1,937,090 ± 53,896; p < 0.001) and an increase of 21.3% in the Butyrate group (1,944,830 ± 53,965) when compared to the Colitis group (p < 0.001) (Figure 10C).