NOS1 and colitis: Analysis of the nuclear area of nNOS-ir neurons showed no difference (p > 0.05) between the Sham (100.7 ± 1.4 μm2), Colitis (103.9 ± 1.5 μm2), and Butyrate (102.5 ± 1.9 μm2) group (Figure 7B).