Analysis of the neuronal profile area of ChAT-ir neurons showed a 14.8% reduction in the area of neurons in the Colitis group (213.5 ± 1.2 μm2) when compared to the Sham group (250.6 ± 4.2 μm2; p < 0.001) and a 15.1% increase in the area of these neurons in the Butyrate group (251.6 ± 1.8 μm2; p < 0.001) when compared to the Colitis group (Figure 7D). This evidence concerns the gene CHAT and colitis.