The number of ChAT-ir neurons per ganglion showed a reduction of 28.3% in the Colitis group (6.1 ± 0.1) when compared to the Sham group (8.6 ± 0.1; p < 0.0001) and an increase of 26.2% in the Butyrate group (8.4 ± 0.1; p < 0.0001), compared to the Colitis group (Figure 6C). Here, CHAT is linked to colitis.