Anti-CHIKV IgM and IgG assays presented a lower sensitivity at earlier time points after the onset of symptoms (≤24.0% for CHIKV IgM assays at 0–4 days, and ≤26.5% for CHIKV IgG assays at 0–10 days; Table 5 and Figure S1), as predicted from the reported kinetics of antibody response following a CHIKV infection [5,6,19,20]. This evidence concerns the gene CD40LG and infection.