We then explored by real-time qPCR the impact of miR-662 overexpression in MDA-MB-231 cells on expression levels of well-established stemness markers that are involved in embryogenesis and cancer –NOTCH1, WNT7b, ZEB1, TCF3, CTNNB1, CD44, CD24, SNAI2, OCT-04, c-MYC, EZH2– [41, 42]. The gene discussed is CD44; the disease is cancer.