NOTCH1 and cancer: Furthermore, miR-662 overexpression in MDA-MB-231 cells substantially increased mRNA expression levels of stemness markers involved in embryogenesis and cancer, such as NOTCH1, WNT7b, ZEB1, TCF3, and SNAI2 (Fig. 5c), reinforcing the notion that miR-662 expression enhanced stem-like properties of BC cells.