Therefore, to confirm the role of B cell-derived IL-10 in LPS-induced ALI, we constructed B cell conditional knockout IL-10 mice and found that B cell-derived IL-10 does not affect the initiation of ALI as manifested by similar infiltration of neutrophils and clinical signs between IL-10B-WT and IL-10B-KO mice following LPS stimulation at day 1. The gene discussed is IL24; the disease is acute respiratory distress syndrome.