Based on the clinical association of ARRB2 and its GSEA in NSCLCs, we investigated the molecular mechanism by which ARRB2 regulated lung cancer progression induced by TLR stimulation and verified the molecular mechanism in ARRB2-knockout (ARRB2KO) lung cancer cells generated by CRISPR/Cas9 gene-editing. The gene discussed is ARRB2; the disease is lung carcinoma.