The results showed that the expression levels of BICC1 were elevated in a high percentage of PAAD tumor tissues (Fig. 1f–h), which was validated by the results of a published single-cell sequencing dataset (Supplementary Fig. 2).29 BICC1 overexpression was strongly correlated with larger tumor size (P = 0.010), regional lymph node involvement (P = 0.008), advanced T stage (P = 0.031) and TNM stage (P = 0.033) (Supplementary Table 1). This evidence concerns the gene BICC1 and neoplasm.