Moreover, HFD-induced cardiac fibrosis and hypertrophy were proved by an expression elevation of β-MyHC, COL-1, and TGF-β1 in the cardiac tissue of DCLK1f/f-HFD group mice, while this increase was not observed in DCLK1lyz-cre group mice (Fig. 2I, J and Supplementary Fig. S2G). This evidence concerns the gene TGFB1 and fibrosis.