In the GBM cell line, we found that the expressions of NLGN3 and NRXN3 were significantly enriched in neurospheres under sphere culture conditions (3-dimensional, 3D condition) compared to typical adherent culture conditions (2-dimensional, 2D condition) in normal media containing 10% FBS (Fig. 3A, B). This evidence concerns the gene NLGN3 and glioblastoma.