CYP11A1 and glioblastoma: Using quantitative reverse transcription PCR (qRT-PCR), we evaluated the expression of various CYP450s in Miyazaki glioblastoma multiforme-1 (MGM-1), Miyazaki glioblastoma multiforme-3 (MGM-3), normal human astrocyte (NHA), and human microglia clone 3 (HMC3) cells (Table 1, Dataset S1), which are different human glial cell lines that we have previously shown to synthesize pregnenolone independently of CYP11A1 (22).